Nucleophosmin Phosphorylation as a Diagnostic and Therapeutic Target for Ischemic AKI.

ABSTRACT: Background Ischemic AKI lacks a urinary marker for early diagnosis and an effective therapy. Differential nucleophosmin (NPM) phosphorylation is a potential early marker of ischemic renal cell injury and a therapeutic target.Methods Differential NPM phosphorylation was assessed by mass spectrometry in NPM harvested from murine and human primary renal epithelial cells, fresh kidney tissue, and urine before and after ischemic injury. The biologic behavior and toxicity of NPM was assessed using phospho-NPM mutant proteins that either mimic stress-induced or normal NPM phosphorylation. Peptides designed to interfere with NPM function were used to explore NPM as a therapeutic target.Results Within hours of stress, virtually identical phosphorylation changes were detected at

SUBMITTER: Wang Z 

PROVIDER: S-EPMC6317607 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature