Structure of the µ-opioid receptor-G_i protein complex.

ABSTRACT: The μ-opioid receptor (μOR) is a G-protein-coupled receptor (GPCR) and the target of most clinically and recreationally used opioids. The induced positive effects of analgesia and euphoria are mediated by μOR signalling through the adenylyl cyclase-inhibiting heterotrimeric G protein G_i. Here we present the 3.5 Å resolution cryo-electron microscopy structure of the μOR bound to the agonist peptide DAMGO and nucleotide-free G_i. DAMGO occupies the morphinan ligand pocket, with its N terminus interacting with conserved receptor residues and its C terminus engaging regions important for opioid-ligand selectivity. Comparison of the μOR-G_i complex to previously determined structures of other GPCRs bound to the stimulatory G protein G_s reveals differenc

SUBMITTER: Koehl A 

PROVIDER: S-EPMC6317904 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature