Therapeutic Potential of Focal Adhesion Kinase Inhibition in Small Cell Lung Cancer.

ABSTRACT: Small cell lung cancer (SCLC) has a poor prognosis. Focal adhesion kinase (FAK) is a non-receptor tyrosine kinase regulating cell proliferation, survival, migration, and invasion, which is overexpressed and/or activated in several cancers, including SCLC. We wanted to determine whether FAK contributes to SCLC aggressive behavior. We first evaluated the effect of FAK small-molecule inhibitor PF-573,228 in NCI-H82, NCI-H146, NCI-H196, and NCI-H446 SCLC cell lines. PF-573,228 (0.1-5 μmol/L) inhibited FAK activity by decreasing phospho-FAK (Tyr397), without modifying total FAK expression. PF-573,228 decreased proliferation, decreased DNA synthesis, induced cell-cycle arrest in G₂-M phases, and increased apoptosis in all cell lines. PF-573,228 also decreased motility in adherent cell

SUBMITTER: Aboubakar Nana F 

PROVIDER: S-EPMC6318062 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature