Acto-myosin force organization modulates centriole separation and PLK4 recruitment to ensure centriole fidelity.

ABSTRACT: The presence of aberrant number of centrioles is a recognized cause of aneuploidy and hallmark of cancer. Hence, centriole duplication needs to be tightly regulated. It has been proposed that centriole separation limits centrosome duplication. The mechanism driving centriole separation is poorly understood and little is known on how this is linked to centriole duplication. Here, we propose that actin-generated forces regulate centriole separation. By imposing geometric constraints via micropatterns, we were able to prove that precise acto-myosin force arrangements control direction, distance and time of centriole separation. Accordingly, inhibition of acto-myosin contractility impairs centriole separation. Alongside, we observed that organization of acto-myosin force modulates specifically

SUBMITTER: Vitiello E 

PROVIDER: S-EPMC6318293 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature