In Vivo PET Tracking of ⁸⁹Zr-Labeled Vγ9Vδ2 T Cells to Mouse Xenograft Breast Tumors Activated with Liposomal Alendronate.

ABSTRACT: Gammadelta T (γδ-T) cells are strong candidates for adoptive immunotherapy in oncology due to their cytotoxicity, ease of expansion, and favorable safety profile. The development of γδ-T cell therapies would benefit from non-invasive cell-tracking methods and increased targeting to tumor sites. Here we report the use of [⁸⁹Zr]Zr(oxinate)₄ to track Vγ9Vδ2 T cells in vivo by positron emission tomography (PET). In vitro, we showed that ⁸⁹Zr-labeled Vγ9Vδ2 T cells retained their viability, proliferative capacity, and anti-cancer cytotoxicity with minimal DNA damage for amounts of ⁸⁹Zr ≤20 mBq/cell. Using a mouse xenograft model of human breast cancer, ⁸⁹Zr-labeled γδ-T cells were tracked by PET imaging over 1 week. To increase tumor antige

SUBMITTER: Man F 

PROVIDER: S-EPMC6318719 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature