Establishment of TP53-knockout canine cells using optimized CRIPSR/Cas9 vector system for canine cancer research.

ABSTRACT:

Background

Genetic engineering technology such as clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system provides a powerful tool for developing disease models and determining gene functions. Recent interests in canine cancer models have highlighted the necessity of developing genetic engineering tools for dogs. In this study, we attempted to generate optimized CRISPR/Cas9 system to target canine tumor protein 53 (TP53), one of the most crucial tumor suppressor genes, to establish TP53 knockout canine cells for canine cancer research.

Results

We constructed CRISPR/Cas9 vectors using each of three TP53 gene-targeting guide RNAs (gRNAs) with minimal off-target potential. After transfection, we obtained several clones of TP53 knockout cells containing "i

SUBMITTER: Eun K 

PROVIDER: S-EPMC6318917 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature