PEDF-derived peptide promotes tendon regeneration through its mitogenic effect on tendon stem/progenitor cells.

ABSTRACT:

Background

Tendon stem/progenitor cells (TSPC) exhibit a low proliferative response to heal tendon injury, leading to limited regeneration outcomes. Exogenous growth factors that activate TSPC proliferation have emerged as a promising approach for treatment. Here, we evaluated the pigment epithelial-derived factor (PEDF)-derived short peptide (PSP; 29-mer) for treating acute tendon injury and to determine the timing and anatomical features of CD146- and necleostemin-positive TSPC in the tendon healing process.

Methods

Tendon cells were isolated from rabbit Achilles tendons, stimulated by the 29-mer and analyzed for colony-forming capacity. The expression of the TSPC markers CD146, Oct4, and nestin, induced by the 29-mer, was examined by immunostaining and western blotting. T

SUBMITTER: Ho TC 

PROVIDER: S-EPMC6318926 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature