Overexpression of SrDXS1 and SrKAH enhances steviol glycosides content in transgenic Stevia plants.

ABSTRACT:

Background

Stevia rebaudiana produces sweet-tasting steviol glycosides (SGs) in its leaves which can be used as natural sweeteners. Metabolic engineering of Stevia would offer an alternative approach to conventional breeding for enhanced production of SGs. However, an effective protocol for Stevia transformation is lacking.

Results

Here, we present an efficient and reproducible method for Agrobacterium-mediated transformation of Stevia. In our attempts to produce transgenic Stevia plants, we found that prolonged dark incubation is critical for increasing shoot regeneration. Etiolated shoots regenerated in the dark also facilitated subsequent visual selection of transformants by green fluorescent protein during Stevia transformation. Using this newly established transformatio

SUBMITTER: Zheng J 

PROVIDER: S-EPMC6318952 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature