N-acetylcysteine decreases malignant characteristics of glioblastoma cells by inhibiting Notch2 signaling.

ABSTRACT:

Background

Glioblastomas multiforme (GBM) is the most devastating primary intracranial malignancy lacking effective clinical treatments. Notch2 has been established to be a prognostic marker and probably involved in GBM malignant progression. N-acetylcysteine (NAC), a precursor of intracellular glutathione (GSH), has been widely implicated in prevention and therapy of several cancers. However, the role of NAC in GBM remains unclear and the property of NAC independent of its antioxidation is largely unknown.

Methods

The mRNA and protein levels of Notch family and other related factors were detected by RT-PCR and western blot, respectively. In addition, intracellular reactive oxygen species (ROS) was measured by flow cytometry-based DCFH-DA. Moreover, cell viability was assess

SUBMITTER: Deng J 

PROVIDER: S-EPMC6319015 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature