A nutrigenetic approach for investigating the chemopreventive effects of black raspberries during the development of preneoplastic esophagi in rats.

ABSTRACT:

Background

Large epidemiological studies have shown that diets high in fruits reduce the risk of esophageal squamous cell carcinoma (ESCC).

Objective

The current study investigated the effects of black raspberries (BRBs) on gene expression during the development of preneoplastic esophagi in rats.

Methods

Using a post-initiation protocol, F344 rats were injected with N-nitrosomethylbenzylamine (NMBA) and then fed either a control diet or 5% BRBs. At weeks 9, 15, and 35, we euthanized subgroups of the rats and collected preneoplastic esophagi to isolate RNA samples for DNA microarray.

Results

Along the development of NMBA-induced preneoplastic esophagi, NMBA injections led to differential expression of 1181 genes comparing to control rats, and dietary BRBs

SUBMITTER: Pan P 

PROVIDER: S-EPMC6319902 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature