Spatiotemporally controlled genetic perturbation for efficient large-scale studies of cell non-autonomous effects.

ABSTRACT: Studies in genetic model organisms have revealed much about the development and pathology of complex tissues. Most have focused on cell-intrinsic gene functions and mechanisms. Much less is known about how transformed, or otherwise functionally disrupted, cells interact with healthy ones toward a favorable or pathological outcome. This is largely due to technical limitations. We developed new genetic tools in Drosophila melanogaster that permit efficient multiplexed gain- and loss-of-function genetic perturbations with separable spatial and temporal control. Importantly, our novel tool-set is independent of the commonly used GAL4/UAS system, freeing the latter for additional, non-autonomous, genetic manipulations; and is built into a single strain, allowing one-generation interrogat

SUBMITTER: Chai A 

PROVIDER: S-EPMC6320068 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature