MITO-Tag Mice enable rapid isolation and multimodal profiling of mitochondria from specific cell types in vivo.

ABSTRACT: Mitochondria are metabolic organelles that are essential for mammalian life, but the dynamics of mitochondrial metabolism within mammalian tissues in vivo remains incompletely understood. While whole-tissue metabolite profiling has been useful for studying metabolism in vivo, such an approach lacks resolution at the cellular and subcellular level. In vivo methods for interrogating organellar metabolites in specific cell types within mammalian tissues have been limited. To address this, we built on prior work in which we exploited a mitochondrially localized 3XHA epitope tag (MITO-Tag) for the fast isolation of mitochondria from cultured cells to generate MITO-Tag Mice. Affording spatiotemporal control over MITO-Tag expression, these transgenic animals enable the rapid, cell-type-specific i

SUBMITTER: Bayraktar EC 

PROVIDER: S-EPMC6320505 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature