High-level expression of Thermomyces dupontii thermo-alkaline lipase in Pichia pastoris under the control of different promoters.

ABSTRACT: In this study, 15 methanol-inducible and 9 constitutive promoters were used to drive the expression of Thermomyces dupontii lipase (TDL) in Pichia pastoris. Of the 15 methanol-inducible promoters, formaldehyde dehydrogenase promoter (P_FLD1) showed the highest efficiency in driving lipase production, followed by alcohol oxidase 1 (P_AOX1) and dihydroxyacetone synthase (P_DAS1) promoters. The maximum lipase activity of transformants with P_FLD1, P_AOX1 and P_DAS1 promoters in 5-l bioreactor was 27,076, 24,159 and 22,342 U/ml, respectively. For the nine constitutive promoters, glycosyl phosphatidyl inositol-anchored protein promoter (P_GCW14) produced the highest amount of lipases in a medium containing gluco

SUBMITTER: Wang J 

PROVIDER: S-EPMC6320703 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature