Regulation of Protein Structural Changes by Incorporation of a Small-Molecule Linker.

ABSTRACT: Proteins have the potential to serve as nanomachines with well-controlled structural movements, and artificial control of their conformational changes is highly desirable for successful applications exploiting their dynamic structural characteristics. Here, we demonstrate an experimental approach for regulating the degree of conformational change in proteins by incorporating a small-molecule linker into a well-known photosensitive protein, photoactive yellow protein (PYP), which is sensitized by blue light and undergoes a photo-induced N-terminal protrusion coupled with chromophore-isomerization-triggered conformational changes. Specifically, we introduced thiol groups into specific sites of PYP through site-directed mutagenesis and then covalently conjugated a small-molecule linker into t

SUBMITTER: Kim Y 

PROVIDER: S-EPMC6321067 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature