Temporal Filtering to Improve Single Molecule Identification in High Background Samples.

ABSTRACT: Single molecule localization microscopy is currently revolutionizing the life sciences as it offers, for the first time, insights into the organization of biological samples below the classical diffraction limit of light microscopy. While there have been numerous examples of new biological findings reported in the last decade, the technique could not reach its full potential due to a set of limitations immanent to the samples themselves. Particularly, high background signals impede the proper performance of most single-molecule identification and localization algorithms. One option is to exploit the characteristic blinking of single molecule signals, which differs substantially from the residual brightness fluctuations of the fluorescence background. To pronounce single molecule signals, w

SUBMITTER: Reismann AWAF 

PROVIDER: S-EPMC6321103 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature