A Metabologenomic Approach Reveals Changes in the Intestinal Environment of Mice Fed on American Diet.

ABSTRACT: Intestinal microbiota and their metabolites are strongly associated with host physiology. Developments in DNA sequencing and mass spectrometry technologies have allowed us to obtain additional data that enhance our understanding of the interactions among microbiota, metabolites, and the host. However, the strategies used to analyze these datasets are not yet well developed. Here, we describe an original analytical strategy, metabologenomics, consisting of an integrated analysis of mass spectrometry-based metabolome data and high-throughput-sequencing-based microbiome data. Using this approach, we compared data obtained from C57BL/6J mice fed an American diet (AD), which contained higher amounts of fat and fiber, to those from mice fed control rodent diet. The feces of the AD mice contained

SUBMITTER: Ishii C 

PROVIDER: S-EPMC6321133 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature