Proteome Investigation of Rat Lungs subjected to Ex Vivo Perfusion (EVLP).

ABSTRACT: Ex vivo lung perfusion (EVLP) is an emerging procedure that allows organ preservation, assessment and reconditioning, increasing the number of marginal donor lungs for transplantation. However, physiological and airflow measurements are unable to unveil the molecular mechanisms responsible of EVLP beneficial effects on lung graft and monitor the proper course of the treatment. Thus, it is urgent to find specific biomarkers that possess these requirements but also accurate and reliable techniques that identify them. The purpose of this study is to give an overview on the potentiality of shotgun proteomic platforms in characterizing the status and the evolution of metabolic pathways during EVLP in order to find new potential EVLP-related biomarkers. A nanoLC-MS/MS system was applied to the p

SUBMITTER: Roffia V 

PROVIDER: S-EPMC6321151 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature