Medicinal Leech CNS as a Model for Exosome Studies in the Crosstalk between Microglia and Neurons.

ABSTRACT: In healthy or pathological brains, the neuroinflammatory state is supported by a strong communication involving microglia and neurons. Recent studies indicate that extracellular vesicles (EVs), including exosomes and microvesicles, play a key role in the physiological interactions between cells allowing central nervous system (CNS) development and/or integrity. The present report used medicinal leech CNS to investigate microglia/neuron crosstalk from ex vivo approaches as well as primary cultures. The results demonstrated a large production of exosomes from microglia. Their incubation to primary neuronal cultures showed a strong interaction with neurites. In addition, neurite outgrowth assays demonstrated microglia exosomes to exhibit significant neurotrophic activities using at least a Tr

SUBMITTER: Raffo-Romero A 

PROVIDER: S-EPMC6321190 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature