Efficient and Stable Delivery of Multiple Genes to Fish Cells by a Modified Recombinant Baculovirus System.

ABSTRACT: The recombinant baculovirus has been widely used as an efficient tool to mediate gene delivery into mammalian cells but has barely been used in fish cells. In the present study, we constructed a recombinant baculovirus containing the dual-promoter cytomegalovirus (CMV) and white spot syndrome virus (WSSV) immediate-early gene 1 (ie1) (WSSV ie1), followed by a puromycin⁻green fluorescent protein (Puro-GFP, pf) or puromycin⁻red fluorescent protein (Puro-RFP, pr) cassette, which simultaneously allowed for easy observation, rapid titer determination, drug selection, and exogenous gene expression. This recombinant baculovirus was successfully transduced into fish cells, including Mylopharyngodon piceus bladder (MPB), fin (MPF), and kidney (MPK); Oryzias latipes sperm

SUBMITTER: Wang Q 

PROVIDER: S-EPMC6321422 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature