Daidzein-Stimulated Increase in the Ciliary Beating Amplitude via an [Cl-]i Decrease in Ciliated Human Nasal Epithelial Cells.

ABSTRACT: The effects of the isoflavone daidzein on the ciliary beat distance (CBD, which is a parameter assessing the amplitude of ciliary beating) and the ciliary beat frequency (CBF) were examined in ciliated human nasal epithelial cells (cHNECs) in primary culture. Daidzein decreased [Cl-]i and enhanced CBD in cHNECs. The CBD increase that was stimulated by daidzein was mimicked by Cl--free NO₃- solution and bumetanide (an inhibitor of Na⁺/K⁺/2Cl- cotransport), both of which decreased [Cl-]i. Moreover, the CBD increase was inhibited by 5-Nitro-2-(3-phenylpropylamino)benzoic acid (NPPB, a Cl- channel blocker), which increased [Cl-]i. CBF was also decreased by NPPB. The rate of [Cl-]i decrease evoked by Cl--free NO₃- solution was enhanced by daidzein. These results suggest that daidzein activates

SUBMITTER: Inui TA 

PROVIDER: S-EPMC6321431 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature