A method of producing genetically manipulated mouse mammary gland.

ABSTRACT:

Background

To obtain a deep understanding of the mechanism by which breast cancer develops, the genes involved in tumorigenesis should be analyzed in vivo. Mouse mammary gland can regenerate completely from a mammary stem cell (MaSC), which enables us to analyze the effect of gene expression and repression on tumorigenesis in mammary gland regenerated from genetically manipulated MaSCs. Although lentiviral and retroviral systems have usually been applied for gene transduction into MaSCs, they are associated with difficulty in introducing long, repeated, or transcriptional termination sequences. There is thus a need for an easier and quicker gene delivery system.

Methods

We devised a new system for gene delivery into MaSCs using the piggyBac transposon vectors and electropora

SUBMITTER: Tagaya H 

PROVIDER: S-EPMC6321679 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature