Mutation analysis by deep sequencing of pancreatic juice from patients with pancreatic ductal adenocarcinoma.

ABSTRACT:

Background

Reliable methods are needed to identify patients with early-stage cancer or high-grade precancerous lesions in the pancreas. Analysis of pancreatic juice to detect somatic mutations could represent one such approach. Here we investigated the concordance between mutations found in the primary tumor and pancreatic juice from the same patient.

Methods

Amplicon-based targeted deep sequencing was performed on samples from 21 patients with pancreatic ductal adenocarcinoma (PDAC) who had undergone Whipple's operation. Mutation profiles were determined in formalin-fixed sections of the primary tumor and in pancreatic juice sampled from the main pancreatic duct during surgery.

Results

Using a cut-off of 3% for variant allele frequency, KRAS mutations were detected i

SUBMITTER: Choi MH 

PROVIDER: S-EPMC6321709 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature