Altered composition and phenotype of mucosal-associated invariant T cells in early untreated rheumatoid arthritis.

ABSTRACT:

Background

Mucosal-associated invariant T (MAIT) cells are innate-like T cells that recognise bacterial metabolites presented by MHC class I-related protein 1 (MR1). Bacterial dysbiosis has been implicated in auto-inflammatory disease development. We investigated MAIT cells in early, untreated rheumatoid arthritis (RA) and spondyloarthritis (SpA) patients.

Methods

Blood and synovial fluid mononuclear cells obtained from patients (SpA/RA) and controls were stimulated with fixed Escherichia coli to provide MAIT ligand. Cells were analysed by flow cytometry and MAIT cells were identified by MR1-5-OP-RU tetramers. Synovial biopsies were studied by confocal microscopy.

Results

Peripheral and synovial CD3⁺ MR1-tet⁺ MAIT cell frequencies were comparable

SUBMITTER: Koppejan H 

PROVIDER: S-EPMC6321723 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature