Developmental effector gene regulation: Multiplexed strategies for functional analysis.

ABSTRACT: The staggering complexity of the genome controls for developmental processes is revealed through massively parallel cis-regulatory analysis using new methods of perturbation and readout. The choice of combinations of these new methods is tailored to the system, question and resources at hand. Our focus is on issues that include the necessity or sufficiency of given cis-regulatory modules, cis-regulatory function in the normal spatial genomic context, and easily accessible high throughput and multiplexed analysis methods. In the sea urchin embryonic model, recombineered BACs offer new opportunities for consecutive modes of cis-regulatory analyses that answer these requirements, as we here demonstrate on a diverse suite of previously unstudied sea urchin effector genes expressed in skeletoge

SUBMITTER: Wang L 

PROVIDER: S-EPMC6321769 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature