Efficient derivation of extended pluripotent stem cells from NOD-scid Il2rg^-/- mice.

ABSTRACT: Recently we have established a new culture condition enabling the derivation of extended pluripotent stem (EPS) cells, which, compared to conventional pluripotent stem cells, possess superior developmental potential and germline competence. However, it remains unclear whether this condition permits derivation of EPS cells from mouse strains that are refractory or non-permissive to pluripotent cell establishment. Here, we show that EPS cells can be robustly generated from non-permissive NOD-scid Il2rg^-/- mice through de novo derivation from blastocysts. Furthermore, these cells can also be efficiently generated by chemical reprogramming from embryonic NOD-scid Il2rg^-/- fibroblasts. NOD-scid Il2rg^-/- EPS cells can be expanded for more than 20 passages with ge

SUBMITTER: Du Y 

PROVIDER: S-EPMC6321811 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature