Rapid generation of gene-targeted EPS-derived mouse models through tetraploid complementation.

ABSTRACT: One major strategy to generate genetically modified mouse models is gene targeting in mouse embryonic stem (ES) cells, which is used to produce gene-targeted mice for wide applications in biomedicine. However, a major bottleneck in this approach is that the robustness of germline transmission of gene-targeted ES cells can be significantly reduced by their genetic and epigenetic instability after long-term culturing, which impairs the efficiency and robustness of mouse model generation. Recently, we have established a new type of pluripotent cells termed extended pluripotent stem (EPS) cells, which have superior developmental potency and robust germline competence compared to conventional mouse ES cells. In this study, we demonstrate that mouse EPS cells well maintain developmental potency

SUBMITTER: Li H 

PROVIDER: S-EPMC6321812 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature