Culture-Independent Analysis of Pediatric Bronchoalveolar Lavage Specimens.

ABSTRACT:

Rationale

The clinical utility of culture-independent testing of pediatric BAL specimens is unknown. In addition, the variability of the pediatric pulmonary microbiome with patient characteristics is not well understood.

Objectives

To compare testing with 16S rRNA gene-based sequencing to conventional cultures of BAL specimens in children Methods: Study subjects were not more than 22 years old and underwent BAL from May 2013 to August 2015 as part of clinical care. DNA extracted from BAL specimens was used for 16S rRNA gene-based analysis, and results were compared with routine cultures from the same samples. Indices of microbial diversity and relative taxon abundances were compared on the basis of subject characteristics (diagnosis and antibiotic use).

Results

From 8

SUBMITTER: Zachariah P 

PROVIDER: S-EPMC6322044 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature