Rapid Identification of Clinically Relevant Mycobacterium Species by Multicolor Melting Curve Analysis.

ABSTRACT: The sustained increase in the incidence of nontuberculous mycobacterial (NTM) infection and the difficulty in distinguishing these infections from tuberculosis constitute an urgent need for NTM species-level identification. The MeltPro Myco assay is the first diagnostic system that identifies 19 clinically relevant mycobacteria in a single reaction based on multicolor melting curve analysis run on a real-time PCR platform. The assay was comprehensively evaluated regarding its analytical and clinical performances. The MeltPro Myco assay accurately identified 51 reference mycobacterial strains to the species/genus level and showed no cross-reactivity with 16 nonmycobacterial strains. The limit of detection was 300 bacilli/ml, and 1% of the minor species was detected in the case of mixed infe

SUBMITTER: Xu Y 

PROVIDER: S-EPMC6322455 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature