A seed resource for screening functionally redundant genes and isolation of new mutants impaired in CO2 and ABA responses.

ABSTRACT: The identification of homologous genes with functional overlap in forward genetic screens is severely limited. Here, we report the generation of over 14000 artificial microRNA (amiRNA)-expressing plants that enable screens of the functionally redundant gene space in Arabidopsis. A protocol was developed for isolating robust and reproducible amiRNA mutants. Examples of validation approaches and essential controls are presented for two new amiRNA mutants that exhibit genetically redundant phenotypes and circumvent double mutant lethality. In a forward genetic screen for abscisic acid (ABA)-mediated inhibition of seed germination, amiRNAs that target combinations of known redundant ABA receptor and SnRK2 kinase genes were rapidly isolated, providing a strong proof of principle for this approa

SUBMITTER: Hauser F 

PROVIDER: S-EPMC6322574 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature