Identification of target genes downstream of semaphorin6A/PlexinA2 signaling in zebrafish.

ABSTRACT:

Background

Semaphorin (Sema)/Plexin (Plxn) signaling is important for many aspects of neuronal development, however, the transcriptional regulation imposed by this signaling pathway is unknown. Previously, we identified an essential role for Sema6A/PlxnA2 signaling in regulating proliferation and cohesion of retinal precursor cells (RPCs) during early eye development. This study used RNA isolated from control, Sema6A-deficient and PlxnA2-deficient zebrafish embryos in a microarray analysis to identify genes that were differentially expressed when this signaling pathway was disrupted.

Results

We uncovered a set of 58 transcripts, and all but 1 were up-regulated in both sema6A and plxnA2 morphants. We validated gene expression changes in subset of candidates that are suggested

SUBMITTER: Emerson SE 

PROVIDER: S-EPMC6322676 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature