Endoplasmic reticulum stress alters ryanodine receptor function in the murine pancreatic β cell.

ABSTRACT: Alterations in endoplasmic reticulum (ER) calcium (Ca²⁺) levels diminish insulin secretion and reduce β-cell survival in both major forms of diabetes. The mechanisms responsible for ER Ca²⁺ loss in β cells remain incompletely understood. Moreover, a specific role for either ryanodine receptor (RyR) or inositol 1,4,5-triphosphate receptor (IP₃R) dysfunction in the pathophysiology of diabetes remains largely untested. To this end, here we applied intracellular and ER Ca²⁺ imaging techniques in INS-1 β cells and isolated islets to determine whether diabetogenic stressors alter RyR or IP₃R function. Our results revealed that the RyR is sensitive mainly to ER stress-induced dysfunction, whereas cytokine stress specifically alters IP_3</

SUBMITTER: Yamamoto WR 

PROVIDER: S-EPMC6322901 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature