Mechanisms of unprimed and dexamethasone-primed nonviral gene delivery to human mesenchymal stem cells.

ABSTRACT: Human mesenchymal stem cells (hMSCs) are under intense study for applications of cell and gene therapeutics because of their unique immunomodulatory and regenerative properties. Safe and efficient genetic modification of hMSCs could increase their clinical potential by allowing functional expression of therapeutic transgenes or control over behavior and differentiation. Viral gene delivery is efficient, but suffers from safety issues, while nonviral methods are safe, but highly inefficient, especially in hMSCs. Our lab previously demonstrated that priming cells before delivery of DNA complexes with dexamethasone (DEX), an anti-inflammatory glucocorticoid drug, significantly increases hMSC transfection success. This work systematically investigates the mechanisms of hMSC transfection and DE

SUBMITTER: Hamann A 

PROVIDER: S-EPMC6322959 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature