Discrimination of normal oral mucosa from oral cancer by mass spectrometry imaging of proteins and lipids.

ABSTRACT: Identification of biomarkers for molecular classification of cancer and for differentiation between cancerous and normal epithelium remains a vital issue in the field of head and neck cancer. Here we aimed to compare the ability of proteome and lipidome components to discriminate oral cancer from normal mucosa. Tissue specimens including squamous cell cancer and normal epithelium were analyzed by MALDI mass spectrometry imaging. Two molecular domains of tissue components were imaged in serial sections-peptides (resulting from trypsin-processed proteins) and lipids (primarily zwitterionic phospholipids), then regions of interest corresponding to cancer and normal epithelium were compared. Heterogeneity of cancer regions was higher than the heterogeneity of normal epithelium, and the distrib

SUBMITTER: Bednarczyk K 

PROVIDER: S-EPMC6323087 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature