Relative contributions of adipose-resident CD146⁺ pericytes and CD34⁺ adventitial progenitor cells in bone tissue engineering.

ABSTRACT: Pericytes and other perivascular stem/stromal cells are of growing interest in the field of tissue engineering. A portion of perivascular cells are well recognized to have MSC (mesenchymal stem cell) characteristics, including multipotentiality, self-renewal, immunoregulatory functions, and diverse roles in tissue repair. Here, we investigate the differential but overlapping roles of two perivascular cell subsets in paracrine induction of bone repair. CD146⁺CD34^-CD31^-CD45^-pericytes and CD34⁺CD146^-CD31^-CD45^-adventitial cells were derived from human adipose tissue and applied alone or in combination to calvarial bone defects in mice. In vitro, osteogenic differentiation and tubulogenesis assays were perfo

SUBMITTER: Wang Y 

PROVIDER: S-EPMC6323123 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature