Preparative separation of minor saponins from Panax notoginseng leaves using biotransformation, macroporous resins, and preparative high-performance liquid chromatography.

ABSTRACT:

Background

Ginsenosides with less sugar moieties may exhibit the better adsorptive capacity and more pharmacological activities.

Methods

An efficient method for the separation of four minor saponins, including gypenoside XVII, notoginsenoside Fe, ginsenoside Rd2, and notoginsenoside Fd, from Panax notoginseng leaves (PNL) was established using biotransformation, macroporous resins, and subsequent preparative high-performance liquid chromatography.

Results

The dried PNL powder was immersed in the distilled water at 50°C for 30 min for converting the major saponins, ginsenosides Rb1, Rc, Rb2, and Rb3, to minor saponins, gypenoside XVII, notoginsenoside Fe, ginsenoside Rd2, and notoginsenoside Fd, respectively, by the enzymes present in PNL. The adsorption charact

SUBMITTER: Liu F 

PROVIDER: S-EPMC6323246 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature