Exploring the unmapped DNA and RNA reads in a songbird genome.

ABSTRACT:

Background

A widely used approach in next-generation sequencing projects is the alignment of reads to a reference genome. Despite methodological and hardware improvements which have enhanced the efficiency and accuracy of alignments, a significant percentage of reads frequently remain unmapped. Usually, unmapped reads are discarded from the analysis process, but significant biological information and insights can be uncovered from these data. We explored the unmapped DNA (normal and bisulfite treated) and RNA sequence reads of the great tit (Parus major) reference genome individual. From the unmapped reads we generated de novo assemblies, after which the generated sequence contigs were aligned to the NCBI non-redundant nucleotide database using BLAST, identifying the closest known

SUBMITTER: Laine VN 

PROVIDER: S-EPMC6323668 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature