EnanDIM - a novel family of L-nucleotide-protected TLR9 agonists for cancer immunotherapy.

ABSTRACT:

Background

Toll-like receptor 9 agonists are potent activators of the immune system. Their clinical potential in immunotherapy against metastatic cancers is being evaluated across a number of clinical trials. TLR9 agonists are DNA-based molecules that contain several non-methylated CG-motifs for TLR9 recognition. Chemical modifications of DNA backbones are usually employed to prevent degradation by nucleases. These, however, can promote undesirable off-target effects and therapeutic restrictions.

Methods

Within the EnanDIM® family members of TLR9 agonists described here, D-deoxyribose nucleotides at the nuclease-accessible 3'-ends are replaced by nuclease-resistant L-deoxyribose nucleotides. EnanDIM® molecules with varying sequences were screened for their activation of huma

SUBMITTER: Kapp K 

PROVIDER: S-EPMC6323716 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature