Tumor-associated neutrophils induce EMT by IL-17a to promote migration and invasion in gastric cancer cells.

ABSTRACT:

Purpose

Epithelial to mesenchymal transition (EMT) can contribute to gastric cancer (GC) progression and recurrence following therapy. Tumor-associated neutrophils (TANs) are associated with poor outcomes in a variety of cancers. However, it is not clear whether TANs interact with the EMT process during GC development.

Methods

Immunohistochemistry was performed to examine the distribution and levels of CD66 + neutrophils in samples from 327 patients with GC. CD66b + TANs were isolated either directly from GC cell suspensions or were conditioned from healthy donor peripheral blood polymorphonuclear neutrophils (PMNs) stimulated with tumor tissue culture supernatants (TTCS) and placed into co-culture with MKN45 or MKN74 cells, after which migration, invasion and EMT were measu

SUBMITTER: Li S 

PROVIDER: S-EPMC6323742 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature