Rapid fine mapping of causative mutations from sets of unordered, contig-sized fragments of genome sequence.

ABSTRACT:

Background

Traditional Map based Cloning approaches, used for the identification of desirable alleles, are extremely labour intensive and years can elapse between the mutagenesis and the detection of the polymorphism. High throughput sequencing based Mapping-by-sequencing approach requires an ordered genome assembly and cannot be used with fragmented, un-scaffolded draft genomes, limiting its application to model species and precluding many important organisms.

Results

We addressed this gap in resource and presented a computational method and software implementations called CHERIPIC (Computing Homozygosity Enriched Regions In genomes to Prioritise Identification of Candidate variants). We have successfully validated implementation of CHERIPIC using three different types of b

SUBMITTER: Rallapalli G 

PROVIDER: S-EPMC6323790 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature