Multiple amino acid substitutions involved in the adaption of three avian-origin H7N9 influenza viruses in mice.

ABSTRACT:

Background

Avian influenza A H7N9 virus has caused five outbreak waves of human infections in China since 2013 and posed a dual challenge to public health and poultry industry. The number of reported H7N9 virus human cases confirmed by laboratory has surpassed that of H5N1 virus. However, the mechanism for how H7N9 influenza virus overcomes host range barrier has not been clearly understood.

Methods

To generate mouse-adapted H7N9 influenza viruses, we passaged three avian-origin H7N9 viruses in mice by lung-to-lung passages independently. Then, the characteristics between the parental and mouse-adapted H7N9 viruses was compared in the following aspects, including virulence in mice, tropism of different tissues, replication in MDCK cells and molecular mutations.

Results

SUBMITTER: Qin J 

PROVIDER: S-EPMC6323857 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature