A Label-free Multicolor Optical Surface Tomography (ALMOST) imaging method for nontransparent 3D samples.

ABSTRACT:

Background

Current mesoscale 3D imaging techniques are limited to transparent or cleared samples or require the use of X-rays. This is a severe limitation for many research areas, as the 3D color surface morphology of opaque samples-for example, intact adult Drosophila, Xenopus embryos, and other non-transparent samples-cannot be assessed. We have developed "ALMOST," a novel optical method for 3D surface imaging of reflective opaque objects utilizing an optical projection tomography device in combination with oblique illumination and optical filters.

Results

As well as demonstrating image formation, we provide background information and explain the reconstruction-and consequent rendering-using a standard filtered back projection algorithm and 3D software. We expanded our app

SUBMITTER: Kerstens A 

PROVIDER: S-EPMC6323867 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature