High-Throughput Stability Screening of Neoantigen/HLA Complexes Improves Immunogenicity Predictions.

ABSTRACT: Mutated peptides (neoantigens) from a patient's cancer genome can serve as targets for T-cell immunity, but identifying which peptides can be presented by an MHC molecule and elicit T cells has been difficult. Although algorithms that predict MHC binding exist, they are not yet able to distinguish experimental differences in half-lives of the complexes (an immunologically relevant parameter, referred to here as kinetic stability). Improvement in determining actual neoantigen peptide/MHC stability could be important, as only a small fraction of peptides in most current vaccines are capable of eliciting CD8⁺ T-cell responses. Here, we used a rapid, high-throughput method to experimentally determine peptide/HLA thermal stability on a scale that will be necessary for analysis of neo

SUBMITTER: Blaha DT 

PROVIDER: S-EPMC6324732 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature