COI1-dependent jasmonate signalling affects growth, metabolite production and cell wall protein composition in arabidopsis.

ABSTRACT:

Background and aims

Cultured cell suspensions have been the preferred model to study the apoplast as well as to monitor metabolic and cell cycle-related changes. Previous work showed that methyl jasmonate (MeJA) inhibits leaf growth in a CORONATINE INSENSITIVE 1 (COI1)-dependent manner, with COI1 being the jasmonate (JA) receptor. Here, the effect of COI1 overexpression on the growth of stably transformed arabidopsis cell cultures is described.

Methods

Time-course experiments were carried out to analyse gene expression, and protein and metabolite levels.

Key results

Both MeJA treatment and the overexpression of COI1 modify growth, by altering cell proliferation and expansion. DNA content as well as transcript patterns of cell cycle and cell wall remodelling markers we

SUBMITTER: Bomer M 

PROVIDER: S-EPMC6324744 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature