Calcium signalling regulates the functions of the bZIP protein VIP1 in touch responses in Arabidopsis thaliana.

ABSTRACT:

Background and aims

VIP1 is a bZIP transcription factor in Arabidopsis thaliana. VIP1 and its close homologues transiently accumulate in the nucleus when cells are exposed to hypo-osmotic and/or mechanical stress. Touch-induced root bending is enhanced in transgenic plants overexpressing a repression domain-fused form of VIP1 (VIP1-SRDXox), suggesting that VIP1, possibly with its close homologues, suppresses touch-induced root bending. The aim of this study was to identify regulators of these functions of VIP1 in mechanical stress responses.

Methods

Co-immunoprecipitation analysis using VIP1-GFP fusion protein expressed in Arabidopsis plants identified calmodulins as VIP1-GFP interactors. In vitro crosslink analysis was performed using a hexahistidine-tagged calmodulin and g

SUBMITTER: Tsugama D 

PROVIDER: S-EPMC6324745 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature