Imaging tissues and cells beyond the diffraction limit with structured illumination microscopy and Bayesian image reconstruction.

ABSTRACT:

Background

Structured illumination microscopy (SIM) is a family of methods in optical fluorescence microscopy that can achieve both optical sectioning and super-resolution effects. SIM is a valuable method for high-resolution imaging of fixed cells or tissues labeled with conventional fluorophores, as well as for imaging the dynamics of live cells expressing fluorescent protein constructs. In SIM, one acquires a set of images with shifting illumination patterns. This set of images is subsequently treated with image analysis algorithms to produce an image with reduced out-of-focus light (optical sectioning) and/or with improved resolution (super-resolution).

Findings

Five complete, freely available SIM datasets are presented including raw and analyzed data. We report methods

SUBMITTER: Pospisil J 

PROVIDER: S-EPMC6325271 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature