Relationship between the structure and composition of rumen microorganisms and the digestibility of neutral detergent fibre in goats.

ABSTRACT:

Objective

This experiment was conducted to compare the structure and composition of ruminal microorganisms in goats with high and low neutral detergent fibre (NDF) digestibility.

Methods

Nineteen crossbred goats were used as experimental animals and fed the same total mixed rations during the 30-day pre-treatment and 6-day digestion trialperiods. All faeces were collected during the digestion period for measuring the NDF digestibility. Then, high and the low NDF digestibility individuals were chosen for the high NDF digestibility group (HFD) and low NDF digestibility group (LFD), respectively. Rumen contents were collected for total microbial DNA extraction. The V4 region of the bacterial 16S rRNA gene was amplified using universal primers of bacteria and sequenced using hig

SUBMITTER: Liu K 

PROVIDER: S-EPMC6325412 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature