Immunophenotypic characterization of sphere-forming cells derived from the human renal cell carcinoma cell line 786-O.

ABSTRACT: Cancer stem cells (CSCs) have been found in many different types of malignant tumors. In our previous study, we found that sphere-forming cells (SFCs) from the renal cell carcinoma (RCC) cell line SK-RC-42 are rich in CSCs. However, our previous reports are based on only one human RCC cell line, which makes it difficult to determine whether the findings from this cell line represent the general mechanisms in human RCC. Therefore, in this study, we attempted to evaluate whether the sphere culture method could enrich for CSCs from another human RCC cell line, 786-O, and to characterize their immunological phenotype. We discovered that a small population of 786-O cells was capable of growing as tumor spheres. The SFCs had many properties similar to CSCs, including greater ability to self-rene

SUBMITTER: Shen C 

PROVIDER: S-EPMC6325489 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature