CRISPR-Cas9 a boon or bane: the bumpy road ahead to cancer therapeutics.

ABSTRACT: Genome editing allows for the precise manipulation of DNA sequences in a cell making this technology essential for understanding gene function. CRISPR/Cas9 is a targeted genome-editing platform derived from bacterial adaptive immune system and has been repurposed into a genome-editing tool. The RNA-guided DNA endonuclease, Cas9 can be easily programmed to target new sites by altering its guide RNA sequence, making this technology easier, more efficient, scalable and an indispensable tool in biological research. This technology has helped genetically engineer animal models to understand disease mechanisms and elucidate molecular details that can be exploited for improved therapeutic outcomes. In this review, we describe the CRISPR-Cas9 gene-editing mechanism, CRISPR-screening methods, thera

SUBMITTER: Ghosh D 

PROVIDER: S-EPMC6325665 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature