Evaluation of strategies for the assembly of diverse bacterial genomes using MinION long-read sequencing.

ABSTRACT:

Background

Short-read sequencing technologies have made microbial genome sequencing cheap and accessible. However, closing genomes is often costly and assembling short reads from genomes that are repetitive and/or have extreme %GC content remains challenging. Long-read, single-molecule sequencing technologies such as the Oxford Nanopore MinION have the potential to overcome these difficulties, although the best approach for harnessing their potential remains poorly evaluated.

Results

We sequenced nine bacterial genomes spanning a wide range of GC contents using Illumina MiSeq and Oxford Nanopore MinION sequencing technologies to determine the advantages of each approach, both individually and combined. Assemblies using only MiSeq reads were highly accurate but lacked contigu

SUBMITTER: Goldstein S 

PROVIDER: S-EPMC6325685 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature