Mapping of a novel clubroot resistance QTL using ddRAD-seq in Chinese cabbage (Brassica rapa L.).

ABSTRACT:

Background

Plasmodiophora brassicae is a soil-borne plant pathogen that causes clubroot disease, which results in crop yield loss in cultivated Brassica species. Here, we investigated whether a quantitative trait locus (QTL) in B. rapa might confer resistance to a Korean P. brassicae pathotype isolate, Seosan. We crossed resistant and susceptible parental lines and analyzed the segregation pattern in a F₂ population of 348 lines. We identified and mapped a novel clubroot resistance QTL using the same mapping population that included susceptible Chinese cabbage and resistant turnip lines. Forty-five resistant and 45 susceptible F₂ lines along with their parental lines were used for double digest restriction site-associated DNA sequencing (ddRAD-seq). High resol

SUBMITTER: Laila R 

PROVIDER: S-EPMC6325862 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature